TY - JOUR ID - 27 T1 - A new method for the introduction of recognition site functionality into polymers prepared by molecular imprinting - synthesis and characterization of polymeric receptors for cholesterol A1 - Whitcombe,M.J. A1 - Rodriguez,M.E. A1 - Villar,P. A1 - Vulfson,E.N. Y1 - 1995/// N1 - J Article WHITCOMBE, MJ, AFRC,INST FOOD RES,DEPT BIOTECHNOL & ENZYMOL,WHITEKNIGHTS RD,READING RG6 6BZ,BERKS,ENGLAND JUL 12 KW - ANALOGUE BUILT POLYMERS KW - RACEMIC-RESOLUTION KW - CHIRAL CAVITIES KW - ENANTIOMERIC RESOLUTION KW - FREE SUGARS KW - BINDING KW - CHROMATOGRAPHY KW - DERIVATIVES KW - SELECTIVITY KW - PERFORMANCE RP - NOT IN FILE SP - 7105 EP - 7111 JF - Journal Of The American Chemical Society VL - 117 IS - 27 N2 - A novel approach to the introduction of recognition site functionality into highly cross-linked polymeric matrices via molecular imprinting has been developed for compounds with single (or multiple, spatially separated) hydroxyl groups. This new methodology relies on the use of the 4-vinylphenyl carbonate ester (1) which functions as a covalently bound template monomer but is easily and efficiently cleaved hydrolytically with the loss of CO2. This results in the formation of a noncovalent recognition site, bearing a phenolic residue, capable of interacting with the ligand (template) through hydrogen bonding. The polymers obtained by this method were shown to bind cholesterol with a single dissociation constant, thus displaying characteristics similar to a true biological receptor or synthetic host. It has also been shown that the phenolic residues introduced into the recognition site play an important role in the interaction with ligand, as evident from the suppression of cholesterol binding by chemical modification of the polymer with acetyl and benzoyl chlorides and by the addition of solvents which tend to disrupt hydrogen-bonding ER -