TY - JOUR ID - 3 T1 - Effect of the solvent on recognition properties of molecularly imprinted polymer specific for ochratoxin A A1 - Turner,N.W. A1 - Piletska,E.V. A1 - Karim,K. A1 - Whitcombe,M. A1 - Malecha,M. A1 - Magan,N. A1 - Baggiani,C. A1 - Piletsky,S.A. Y1 - 2004/// N1 - J Article Turner, NW, Cranfield Univ, Inst Biosci & Technol, Silsoe MK45 4DT, Beds, England n.turner.1999@cranfield.ac.uk DEC 15 KW - molecular imprinting KW - polymer conformation KW - ochratoxin A KW - recognitiom KW - sensor KW - SPE KW - MOBILE-PHASE KW - MYCOTOXINS KW - RESOLUTION RP - NOT IN FILE SP - 1060 EP - 1067 JF - Biosensors & Bioelectronics VL - 20 IS - 6 N2 - A molecularly imprinted polymer specific for the mycotoxin ochratoxin A has been synthesised using a non-covalent approach. The polymer has shown an excellent affinity and specificity for the target template in aqueous solutions. The binding experiments, NMR study and molecular modelling have proven that the template recognition by polymer originates from the shape complementarity of binding sites. The binding mechanism is critically depended on factors that affect the polymer conformation. Thus the variation in buffer concentration, pH and presence of organic solvent, which affect the polymer swelling or shrinking, had a profound effect on the polymer recognition properties. (C) 2004 Elsevier B.V. All rights reserved AD - Cranfield Univ, Inst Biosci & Technol, Silsoe MK45 4DT, Beds, England. Inst Food Res, Colney NR4 7UA, Norfolk, England. Univ Turin, Dipartimento Chim Analit, I-10125 Turin, Italy. ER -