Abstract: The technology of molecular imprinting of polymers (MIPs) was used to obtain optical sensors for nucleotides. The methacrylates were used as functional monomers and 1,3-diphenyl-6-vinyl-1H-pyrazole-[3,4-b]-quinoline (PAQ) was used as a fluorescent receptor and the nucleotides: guanosine 3GǦ,5GǦ-cyclic monophosphate (cGMP) and adenosine 3GǦ,5GǦ-cyclic monophosphate (cAMP) were a template. The optical sensor obtained as a thin-layer polymeric films was investigated by steady-state fluorescence spectroscopy. The calculated association constant of the cGMP imprinted sensor is K_a-á=-á1.7-á+ù-á10⁴-áM^-1 and of cAMP imprinted sensor K_a-á=-á9.9-á+ù-á10⁴-áM^-1
Template and target information: guanosine 3',5'-cyclic monophosphate, cGMP, adenosine 3',5'-cyclic monophosphate, cAMP
Author keywords: fluorescence spectroscopy, MIPs, nucleotides, optical sensors, pyrazolequinolines